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 Microtubule behaviour in live syncitial embryos
Spindle assembly during mitosis in a syncitial Drosophila embryo.
Detail of one cell where recording of GFP-alphaTubulin84B reveals the process of spindle assembly during two consecutive cell cycles, 10 and 11. After the first mitosis, the single telophase aster splits into two that migrate symmetrically around the nuclear envelope in preparation for the following mitosis.
Spindle assembly during mitosis in a syncitial Drosophila embryo.
Recording of GFP-alphaTubulin84B reveals spindle assembly in a subset of syncitial mitosis during cycles 10 to 11. Mitosis is propagated as a wave along the monolayer of nuclei located under the cell membrane. Note the reduction in spindle length from cycle 10 to 11.
Spindle assembly during mitosis in a syncitial Drosophila embryo.
Recording of GFP-alphaTubulin84B reveals spindle assembly in a subset of syncitial mitosis during cycles 10 to 11. Note the formation of the central spindle during telophase. After the central spindle is disrupted, splitting of the aster into two and migration of these around the nuclear envelope can be observed.
Spindle assembly and chromosome segregation during mitosis in a syncitial Drosophila embryo.
GFP-alphaTubulin (in green) 84B and Histone2-RFP (in red) reveal the behaviour of microtubules and chromosomes respectively in a subset of syncitial mitosis at cycle 10. Chromosome condensation occurs immediately after nuclear envelope breakdown, revealed as the entry of GFP-alphaTubulin 84B signal into the nuclear area. Chromosome congression to the metaphase plate and segregation during anaphase can be observed. At telophase the central spindle is formed between the separating daughter nuclei.
Spindle assembly during mitosis in a syncitial Drosophila embryo.
GFP-alphaTubulin84B behaviour observed at low magnification reveals the mitotic waves that originate from anterior and posterior poles and converge in the middle of the embryo.
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