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 Microtubule behaviour in live spermatocytes
Spindle assembly during meiosis I in primary Drosophila spermatocytes.
Recording of GFP-alphaTubulin84B in primary Drosophila spermatocytes reveals the location of two diametrically opposed asters around the nuclear envelope before meiosis I. Upon nuclear envelope breakdown, indicated by the sudden entry of GFP signal into the nuclear area, spindle assembly takes place. Intranuclear microtubules form a bipolar structure that connect the asters at the two poles. Extranuclear microtubules derived from both asters expand toward the cortex and converge in the equatorial plane of the cell.
Spindle assembly during meiosis I in primary Drosophila spermatocytes.
Recording of GFP-alphaTubulin84B in primary Drosophila spermatocytes reveals the behaviour of intra- and extranuclear microtubules during the first stages of spindle assembly. Later, at anaphase I, intranuclear microtubules contacting the chromosomes shorten, whereas extranuclear microtubules expand towards the cortex and converge at the position where the cytokinesis furrow will form.
Spindle assembly and chromosome behaviour during meiosis I in primary Drosophila spermatocytes.
Recording of GFP-alphaTubulin84B (in yellow) and Histone2-YFP (in red) reveal microtubule and chromosome behaviour during spindle assembly in meiosis I. During prometaphase I bivalent chromosomes interact with astral microtubules and congress to the metaphase I plate.
Spindle assembly and chromosome behaviour during meiosis I in primary Drosophila spermatocytes.
Recording of GFP-alphaTubulin84B (in yellow) and Histone2-YFP (in red) reveal microtubule and chromosome behaviour during spindle assembly and division in meiosis I. During anaphase I homolog chromosomes separate and migrate to opposite poles as kinetochore microtubules shorten.
Spindle assembly and central spindle formation during meiosis I in primary Drosophila spermatocytes.
Recording of GFP-alphaTubulin84B in a primary Drosophila spermatocyte from the onset of meiosis I to telophase I. The different sets of microtubules can be tracked all throughout the cell cycle. Note how the network of astral microtubules expands during anaphase I along the cortex and how the signal increases after chromosome segregation in the central spindle area.
Spindle assembly and central spindle formation during meiosis I in primary Drosophila spermatocytes.
Recording of GFP-alphaTubulin84B in a primary Drosophila spermatocyte from prometaphase I to the end of cytokinesis I. After spindle elongation at anaphase B the plasma membrane invaginates in the equator of the cell and the cleavage furrow progresses squeezing the central spindle. After completion of cytokinesis the daughter cells remain attached by a GFP positive structure, the midbody.
Central spindle formation and cytokinesis in primary Drosophila spermatocytes.
Recording of GFP-alphaTubulin84B in a primary Drosophila spermatocyte from metaphase I I to the end of cytokinesis I. Note how spindle microtubules shorten as chromosomes segregate to the poles. Central spindle becomes obvious after spindle elongation at anaphase B, and furrow formation occurs afterwards at the position where astral microtubules where touching the cortex. Aster splitting occurs before cleavage constriction is complete.
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