Asymmetric mitosis in larval neuroblasts


Drosophila larval neuroblasts (NBs) are stem cells that undergo asymmetric division producing a new stem cell and a small differentiating cell called ganglion mother cell (GMC). Asymmetric division in larval NBs is characterised by an asymmetric pattern of spindle asesmbly, whereby one of the centrosomes, located apically, retains microtubule polymerisation activity throughout the cell cycle, while the other is transiently downregulated and moves all over the cytoplasm, being able to polymerise microtubules only shortly before mitosis. In this section, time-lapse confocal microscopy videos of several microtubule and centrosome fluorescent markers reveal this process.
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