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 Introduction

 

During cell division, the network of microtubules reorganizes into a highly dynamic bipolar structure called spindle that segregates the condensed chromosomes faithfully to the daughter cells. The process by which spindle assembles may show strong variations among different cell types within the same organism. Drosophila is an excellent system for studing such variability on spindle morphogenesis in vivo. Syncitial embryos are amenable to time-lapse imaging of hundreds of synchronously dividing spindles. Other cell types such as spermatocytes and neuroblasts differ in their design, allowing identification of common core mechanisms of spindle assembly. All these cell types can be followed by confocal fluorescent microscopy using flies expressing GFP markers for microtubules, centrosomes and chromosomes. This collection of videos is aimed to illustrate spindle morphogenesis in different Drosophila tissues and provide live imaging data that will help modelize spindle assembly in animal cells. This project has been financed by COMBIO LSHG-CT-2004-503568 and performed by E. Rebollo, J. Reina and S. Llamazares at the laboratory of C. Gonzalez (Parc Cientific de Barcelona, IRBB). This web site has been created by Raúl Gómez at CRG, Barcelona
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